Most Popular Books by Sean Chen

Don''t miss this exciting sci-fi prequel graphic novel of the highly anticipated Warner Bros. & Legendary motion picture, Pacific Rim directed by Guillermo del Toro! Chronicling the very first time Earth is menaced by incredible monsters known as Kaiju, these inhuman beasts rise from the ocean depths and threaten to extinguish all mankind! Witness the race to develop massive robot fighting machines called Jaegers, each one controlled simultaneously by two pilots whose minds are locked in a neural bridge. This action-packed tale features many of the key characters from the film as we follow them in their early careers. Witten by Pacific Rim screenwriter himself, Travis Beacham, and with del Toro''s hands-on supervision, this volume is beautifully illustrated by Sean Chen, Yvel Guichet, and Pericles Junior; inks by Steve Bird and Mark McKenna; and fully painted cover by superstar artist Alex Ross. From the Hardcover edition.

Here''s a delightful collection of fairy-tale short stories written by children for children. A sheep with two heads. A fish who wants to be an adventurer. An inseparable sheep and a fish. A sad moon who learns to accept life. An ugly fish with three eyes. A baking sheep. From the depth of the imagination of young readers who take their turn at storytelling with wonderful hand-drawn illustrations by Alain Boulanger "Hilarious, engaging and imaginative "

When Wolverine rebuffs the reconstituted Weapon X program, management is forced to settle for second best -- but Deadpool''s first assignment may test the limits of his loyalty.

Endogenous retroviruses (ERVs) constitute a significant fraction of mammalian genomes, but their impact on host biology remains poorly understood. One group of ERVs, murine ERV with leucine tRNA primer (MERVL), is highly expressed during the 2-cell (2C) stage of mouse preimplantation development but is silenced thereafter. While active, MERVL-derived cis-regulatory elements drive expression of hundreds of host genes, including “chimeric” isoforms with exonized MERVL sequences. Remarkably, loss of a single miRNA miR-34a in pluripotent stem cells is sufficient to derepress MERVL and imbue expanded fate potential reminiscent of totipotent 2C blastomeres. Using bioinformatic prediction and reporter assays, I identified gata2 as the primary target of miR-34a that mediates MERVL derepression in pluripotent stem cells. While miR-34a is required for MERVL silencing in pluripotent stem cells, it is dispensable for repressing MERVL during preimplantation development. To evaluate the role of MERVL in vivo, I applied a candidate approach to assess the role of one MERVL-driven chimeric gene tead4:MT2B1. Loss of tead4:MT2B1 results in compensatory upregulation of canonical tead4 transcripts, suggesting that the chimeric isoform functions redundantly in development. In order to facilitate efficient in vivo screening of additional candidates, I developed a high-throughput electroporation-based genome editing technique called CRISPR RNP Electroporation of Zygotes (CRISR-EZ). Compared to previous methodologies, CRISPR-EZ offers significant advantages in throughput, cost, and simplicity. Altogether, I have elucidated a molecular axis involved in the regulation of MERVL and fate potency, setting the stage for further in vivo characterization using improved genome editing tools.