New Releases by Maria Rius

Abstract: Decitabine (5-aza-2′-deoxycytidine) is a DNA methyltransferase inhibitor and an archetypal epigenetic drug for the therapy of myeloid leukemias. The mode of action of decitabine strictly depends on the incorporation of the drug into DNA. However, DNA incorporation and ensuing genotoxic effects of decitabine have not yet been investigated in human cancer cell lines or in models related to the approved indication of the drug. Here we describe a robust assay for the quantitative determination of decitabine incorporation rates into DNA from human cancer cells. Using a panel of human myeloid leukemia cell lines we show appreciable amounts of decitabine incorporation that closely correlated with cellular drug uptake. Decitabine incorporation was also detectable in primary cells from myeloid leukemia patients, indicating that the assay is suitable for biomarker analyses to predict drug responses in patients. Finally, we also used next-generation sequencing to comprehensively analyze the effects of decitabine incorporation on the DNA sequence level. Interestingly, this approach failed to reveal significant changes in the rates of point mutations and genome rearrangements in myeloid leukemia cell lines. These results indicate that standard rates of decitabine incorporation are not genotoxic in myeloid leukemia cells

The following thesis presents a stability study of a floating wind turbine platform, composed by two concentric cylinders, for offshore application. The theoretical bases of the linear theory and the derivation of the equation of motion are introduced in the second chapter. The following chapter resumes the results of the research of the optimum dimensions as well as the results of the simulation of the equation of motion of the spar with and without inertial plate. The addition of the inertial plate and the variation of its porosity (depending on the frequency of the incident wave) help to stabilize the structure in a large frequency bandwidth, due to the increment of the added mass coefficient and the consequent reduction of the resonance frequency. With the collaboration of the Centre of Nanotechnology and Smart Materials, CeNTI, a solution to simulate the porosity of the inertia plate is proposed as well as the material for the body of the spar and the plate and all the necessary corrosion treatments due to the seawater. Finally, the future work and all the conclusions of the study are explained in the last chapter.

The wolf gives his own outlandish version of what really happened when he tangled with the three little pigs.

Two young children explore the changes that occur in nature during the fall season.

Illustrates children participating in a variety of activities that characterize winter. Includes guidelines to aid parents and teachers in teaching children about winter.

This animated video uses scripture, history, and imagination to create a portrait of what life might have been like for Jesus as he was growing up in the Holy Land of ancient times.

A simple explanation of what grandparents are and their place in the family. Includes a guide for parents and teachers.