Best Selling Books by Nicole Edwards

Nicole Edwards is the author of Unraveling - Unhinged Book 2 (2014), Primal Instincts (2023), Sawyer (2015), Braydon (2014), Ethan (2014), Primal Instincts: Volume 1 (2023).

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Unraveling - Unhinged Book 2

release date: Sep 25, 2014
Unraveling - Unhinged Book 2
Secrets and lies... No good ever comes of them. My life is proof. For eleven years, I''ve suffered from a suspicion that has waged a war inside my head, filled me with rage. Left me feeling helpless. But then she came along. The darkness continues to consume me, but when I''m with her, I can ignore it, push it away. Even if only for a little while. Payton calms me. She manages to quiet the noise in my head. But the secret is still there, still continuing to plague me day after day. Yet I still can''t bring myself to tell her even though she deserves to know. She needs to know. I love her. And I''ll do anything to protect her. Although I''ve found some peace, my life continues to unravel, no matter how hard I try to stop it. My name is Sebastian Trovato and this is the continuation of my story.

Primal Instincts

release date: Apr 13, 2023
Primal Instincts
As though dating three men isn''t complicated enough, now there are new feelings to decipher and another threat creating chaos. Only this time, my stalker has stepped up his game, and his actions send the men in my life into protective mode. But not before I learn that they''ve been lying to me since the day I met them. These aren''t little white lies, either. Or ones of omission, no matter what Creed says. These are the kind of lies used to package up secrets that have the ability to alter the entire foundation of our relationship. This time, I''m not looking for space. I''m not walking away. I want answers, and they''re going to give them to me. What I don''t realize is that getting them may very well set us on another course entirely.

Sawyer

release date: Mar 02, 2015
Sawyer
From New York Times and USA TODAY bestselling indie author Nicole Edwards comes the seventh book in her Alluring Indulgence series in which Sawyer, a known womanizer, wants to change his ways when a woman from his past shows up and refuses to fall for him—not after what happened in high school... Sawyer Walker has never run into problems with women. The female population tends to swarm toward him, in fact, and he’s never had a complaint. But there’s one woman Sawyer has always had his eye on. A woman who won’t give him the time of day. In recent months, it would seem that Kennedy Endsley is opening up to Sawyer, but she’s got a few surprises in store for him. Because, as hard as she finds it to resist the handsome Walker brother, she won’t give in easily. Not when her heart is on the line.

Braydon

release date: Nov 24, 2014
Braydon
From New York Times and USA TODAY bestselling author Nicole Edwards comes the sixth searing adventure in her Alluring Indulgence erotic romance series, which follows the seven Walker brothers and their sizzling-hot love interests! Braydon Walker has always shared everything with his identical twin brother Brendon—hopes, dreams, career choices, and yes, even women. Known for tag-teaming their love interests in every way imaginable, Braydon is surprised and unnerved when he finds himself wanting to keep their newest lover all to himself. Will the twins’ relationship evolve to fit this new mold, or will Braydon’s desire for independence tear them apart?

Ethan

release date: May 16, 2014
Ethan
From New York Times and USA Today bestselling indie author Nicole Edwards comes the fifth installment in her Alluring Indulgence series that asks the question: how long did it take you to find your place in the world? For Ethan Walker, going through the motions of life, keeping his distance, and never getting attached has become the mantra he lives by. Success is measured by how many hours he can work and how little attention he can draw. That was until he landed on Beau Bennett’s radar. Until Beau, Ethan had never met a man who could get him to question his own logic and even consider taking a different path. Beau Bennett knows exactly who he is—now. It might’ve taken him some time to figure it out, but now that he has his bearings, Beau has set his sights on the mysteriously sexy Ethan Walker. Pursuing someone has never been his style, but something is telling him to keep at it because the end result is going to be worth it. Now that they’ve found one another, how long will it take Ethan and Beau to accept that their place in the world is...together?

Primal Instincts: Volume 1

release date: Feb 03, 2023
Primal Instincts: Volume 1
What twenty-four-year-old recent college graduate would sign up to uncover a secret kink club rumored to be hidden within the walls of a multi-billion dollar conglomerate? Me. Why? Well, that''s easy. I''ve spent my entire life with my nose to the grindstone, doing the right thing, focusing on my future, and now I''m bored, which isn''t a good thing when you have an insatiable curiosity. I probably should''ve considered the type of bait needed for a fishing expedition of this magnitude when I applied for the job at Primal Instincts, LLC, looking to uncover the secret world that exists only on the whispers of those who''ve never so much as glimpsed the dark, seductive inner workings because those who had wouldn''t dare reveal anything. Now that I''m through the door, I''ve got my determination and single-minded focus to keep me moving forward. I will figure it out if for no other reason than to say I did. Provided I don''t get sidetracked by not one, not two, but three men who are ridiculously good at keeping secrets.

Office Intrigue

release date: Dec 05, 2017

Jared

release date: Sep 10, 2016
Jared
This novel crosses over with Nicole''s Dead Heat Ranch series. It can be read as a standalone. Jared Walker is a single dad who knows what it''s like to be burned by a woman. His ex-wife tormented him in a way that''s unforgivable. It makes sense that Jared doesn''t trust women. He''s not looking for love, but when his cousin Travis puts Jared in charge of the Walker family reunion, Jared just might find himself in over his head. In more ways than one. Hope Lambert has one goal in life. To keep Dead Heat Ranch prospering. From sun up to sun down, she has a single-minded focus working alongside her four sisters, the job isn''t always easy, but it''s definitely worth it. Hope is convinced she needs nothing more than what''s right in front of her. Until he walks into the stable. It''s easy to think you know what''s best for you. That is until your heart is put to the test. What happens when the cautious cowgirl and the sexy single dad come face to face? The chemistry threatens to scorch them both. Giving in is easy. Not falling in love is the hard part.

Naughty Holidays 2015

release date: Dec 01, 2015
Naughty Holidays 2015
Because Naughty Holidays can be oh so Nice... The readers voted and the winners for the 2015 holiday book are: Alluring Indulgence: Travis, Kylie, Gage; Sniper 1 Security: RT and Z; Club Destiny: Luke, Sierra, Cole. Come and see how they are heating up the holidays this year! The Walker brothers are at it again... Travis Walker won’t deny he has a competitive spirit, and this year, when his brothers decide to go all out with the holiday decorations, Travis is intent on winning this free-for-all. What’s the best gift you can get for the man you love? Ryan Trexler is hell-bent on getting his husband the best Christmas gift. One that will make Z think about him all year. Only he doesn’t have the slightest clue what to get him. Spend New Year’s at the hottest fetish club in town Luke McCoy has been known to throw a good party, but this year, he wants the New Year’s celebration at Devotion to be the best yet.

Intrigued Out of the Office

release date: Dec 05, 2017
Intrigued Out of the Office
Having spent the last two and a half months with my bosses, the thought of spending the holidays alone isn’t ideal. Unfortunately, that’s exactly what I feared I would be doing. Or so I thought. I probably should’ve learned long ago not to underestimate the men I work for. They do have a way of surprising me when I least expect it. While I find myself immersed in a lifestyle I am still getting familiar with, spending time with my bosses is proving to be more than I originally anticipated. Not only am I experiencing what it means to offer my submission freely, I’m also experiencing something else. Something that feels a lot like love. Until they switch things up and make me choose between them. That’s not something I’m capable of doing. After all, not only am I falling in love with one of my bosses, it appears my heart already belongs to two. Except one of those two seems to be rejecting me and I’m at a loss. If only I could let things go back to the way they were in the beginning, return to our original arrangement. If I could do that, it might work out. Too bad my heart doesn’t seem to be listening to reason.

Reckless

release date: Oct 14, 2015
Reckless
He''s a walking contradiction... Cam Strickland, one of the four owners of Pier 70 Marina, has earned the nickname his friends gave him years ago. Reckless. Sure. By definition. When it comes to fun, Cam hasn''t yet met a challenge he wouldn''t accept. In other areas, he''s likely far too cautious for his own good. He''s his complete opposite... Gannon Burgess, CEO of Burgess Entertainment, one of the top video game producers in the world, finds himself smack in the middle of the last place he would ever expect to be. But Gannon only thinks he is out of his element standing in the small marina office. Then... he meets Cam. Rumor is opposites attract. Sparks fly when this sexy, tattooed bad boy comes face to face with this buttoned-up gamer in glasses. Can Cam and Gannon push one another out of their comfort zones? Or -- thanks to the events that have shaped their lives -- will the time they spend together prove to be just another reckless adventure? This one involving their hearts.

Zane

release date: May 16, 2014
Zane
Vanessa must learn to open her heart to the one man with the key to hers in this sexy ebook from New York Times and USA TODAY bestselling author Nicole Edwards. Vanessa Carmichael will never forget that horrible day for as long as she lives. With Zane fully on the mend from the brutal attack he suffered right in her very own yard, she knows she should be grateful, which she is. She also knows that walking away from Zane might be the only way to keep him safe. Despite her denial, V knows she can’t trust her own heart, especially when it comes to the youngest Walker brother, but he’s not the kind of man who will walk away when there is something he wants. He wants her even if it isn’t safe for him, and she’s not sure she is strong enough to walk away. Zane Walker might have been brutally attacked and after months in the hospital, walked away with his life spared, but now that he’s out of the hospital, he has only one goal in mind. Getting the girl. From the first time he kissed her, Zane knew V was the one for him. He also knew that getting her wasn’t going to be as easy as he hoped. She’s stubborn and the walls she has erected around her heart are nearly impenetrable. However, there’s one thing about Zane—he never gives up. And he’s not about to start now. Can V learn to open her heart to the one man who is desperate to get past those walls she has erected, or will she walk away to protect him? How long will Zane allow her to deny what he knows is growing between them? Or is the only option to obscure the underlying emotions with spine-tingling lust? When V and Zane come together, smoke detectors go on the fritz, but when they bring Zane’s best friend, Beau, into the mix, extra fire extinguishers are needed.

Travis

release date: Jul 30, 2013
Travis
Travis Walker, the oldest of the seven Walker brothers, has been referred to as moody and mysterious, and for the most part, he would agree. Until recently, he spent his days helping his brothers run the family business. Now, he dedicates his time and energy to building a resort, Alluring Indulgence, that caters to fetishes and fantasies. And despite his attempt to work himself into the ground, Travis has never denied-- at least not to himself-- that something is missing.

Wait for Morning

release date: Apr 07, 2015
Wait for Morning
Trace Kogan will go to any length to protect those who need protecting. That is the business he is in; the business his father, Casper Kogan, started with Bryce Trexler more than thirty-five years ago. Trace spends day and night eliminating threats to clients, so when the danger hits too close to home, he won''t let anything come between him and the woman who desperately needs his help. His only fear, the physical reaction he has to Marissa may pose a bigger danger to them both than the ghost that they find themselves up against. Marissa Trexler has spent the last twelve months of her life in hiding, tucked away in one safe house after another for her own protection. The threat to her is real even if the reason is still unknown. Strangely, whoever is after her seems to find her no matter where she goes, leading her family to believe it''s an inside job. So when the decision is made that Trace will provide personal protection, she can''t say no, even if being close to him adds another level of danger... and this one is to her heart.

Lent...

release date: Feb 28, 2019
Lent...
Lent is the season on the Christian liturgical calendar where we set aside time to set our focus on the events leading up to the cross. This devotional journal hopes to serve as an aide to daily time with God during the practice of fasting and praying. This devotional is in no way intended to be the sum total of theological thought on the matter, however it is a sacrificial offering of insight lead by the Spirit of God. This devotional includes 40 days of scripture, prayer focus and a brief commentary for consideration. There is also space provided for thoughts, notes and prayers.

Soft Skills Versus Hard Skills

release date: Sep 05, 2022
Soft Skills Versus Hard Skills
Employers are looking for candidates that have soft skills and hard skills. Soft Skills versus Hard Skills help identify what people need to gain meaningful employment. Having a career readiness certificate will provide a solid foundation for a rewarding career.

Fearless

release date: Apr 10, 2016
Fearless
He''s the one who walked away... Dare Davis, one of the four owners of Pier 70 Marina, has been living the past fifteen years of his life not taking risks. He considers himself fearless because he refuses to let anyone close enough to hurt him. He''s had his heart broken before, he''s not looking for it to happen again. He''s the one who let love slip through his fingers... Noah Pearson, thirteen-year veteran of the Austin Fire Department, finds himself being roped into a favor by his mischievous stepsister. When Milly invites him to come along on a cruise to the Caribbean, he finds himself giving in. What he doesn''t know is that Milly''s playing matchmaker. However, Milly''s surprise backfires when Noah finds himself front and center with the man who slipped through his fingers fifteen years ago. Some people believe in second chances. Things heat up when the mischievous, fun-loving bad boy comes face to face with this smoking hot firefighter. Now that Dare and Noah find themselves sharing a room on a week-long cruise, can they find a way to keep the peace and ignore their past? Or - thanks to the decisions they made long ago - will they come full circle, picking right back up where they left off?

Speechless

release date: May 26, 2016
Speechless
He''s suffering in silence... Teague Carter, one of the four owners of Pier 70 Marina, has spent his life feeling rejected. To the point that he has never allowed himself to get close to anyone. He prefers it that way, in fact. But he refuses to believe that his destructive behavior might be something more than rebellion. He''s unable to speak... Hudson Ballard, Pier 70''s mechanic, has been fighting his attraction to Teague for years. He has stood by and watched the kid get more and more out of control, but this time is the last. If Teague wants to use sex to escape his problems, Hudson is certainly willing to be the one to show him that he''s good with more than just his hands. Sometimes words aren''t necessary. There are fireworks when this out-of-control bad boy agrees to no strings with this tatted up, dominating mechanic. Can Teague and Hudson keep their emotions from getting involved in their arrangement? Or -- thanks to the demons from their pasts -- will the darkness steal from them before they have a chance?

Their Fairy Princess

release date: Dec 22, 2019
Their Fairy Princess
Have you ever felt ... incomplete? As though happily ever after is a fleeting, unattainable concept? Not that I haven''t had memorable experiences because I have. Plenty of them. It''s more like what''s necessary to check off all the boxes for a successful HEA hasn''t been defined yet. At least not for me. To say my desires-not to mention my expectations-are complicated would be putting it mildly. It''s bad enough I''m in love with my best friend despite the fact we can''t give each other what we both need: a Dominant who can tame our inner submissives.Then the unexpected happens. Secrets are revealed, danger unfolds, decisions are made. And it''s during the ensuing roller coaster ride that five peoples'' lives are forever changed. Including mine.

Beautifully Brutal

release date: Jun 16, 2015
Beautifully Brutal
Sometimes love isn''t beautiful... Sometimes the only way to describe it is beautifully brutal.If you think you know him... You''re wrong. If you think you know his family... Wrong again. If you think you know his story... You don''t. If you did, you''d likely wonder how the hell a man like Maximillian Adorite managed to make it to this point in his life. At twenty-nine, he''s experienced more than most people could only ever imagine, all thanks to his family. Organized crime they call it. Mafia. No matter how they spin it, it comes down to one thing. Max gets what he wants. Except for the one thing he wants most. Her name is Courtney Kogan. And now that he''s had a taste of her, Max knows he''ll never be able to let her go.

Giollachríst

release date: Jul 30, 2022
Giollachríst
Giollachríst: Lighting the Candle brilliantly weaves historical fact with paranormal mystery and spirituality, portraying diverse elements such as the supernatural, humor, autism, prejudice, and Irish culture. Peter O''Sullivan was a teenage Irish immigrant whose life came to a tragic end after his family settled in the Blue Ridge Mountains of Virginia and worked for the Catawba Iron and Coal Company. For more than a hundred years, Peter''s spirit has been anchored in his Virginia home between this world and Heaven. He longs to let go and is unable to communicate with anyone until he meets Emma Roberts. Emma is a shy teenager who moves into Peter''s house, recently renovated in 2018. Growing up on the autism spectrum, she struggles with challenges of her own even though surrounded by a loving family. Peter and Emma''s futures are changed when she discovers she is the only person who can see and communicate with Peter''s spirit. Each becomes the strength the other so desperately needs. Will Peter''s secrets and a hidden family history hold the answer to his and Emma''s profound connection? The valley''s forgotten past is reawakened as answers come to light.

Because Naughty Holidays Can Be Oh So Nice 2015

release date: Dec 01, 2015
Because Naughty Holidays Can Be Oh So Nice 2015
The readers voted and the winners for the 2015 holiday book are: Alluring Indulgence: Travis, Kylie, Gage; Sniper 1 Security: RT and Z; Club Destiny: Luke, Sierra, Cole. Come and see how they are heating up the holidays this year! The Walker brothers are at it again... Travis Walker won''t deny he has a competitive spirit, and this year, when his brothers decide to go all out with the holiday decorations, Travis is intent on winning this free-for-all. What''s the best gift you can get for the man you love? Ryan Trexler is hell-bent on getting his husband the best Christmas gift. One that will make Z think about him all year. Only he doesn''t have the slightest clue what to get him. Spend New Year''s at the hottest fetish club in town Luke McCoy has been known to throw a good party, but this year, he wants the New Year''s celebration at Devotion to be the best yet.

This World Below

release date: Dec 21, 2024
This World Below
In this anticipated prequel to Lighting the Candle, Caitlín and Kieran O''Sullivan have started a family on the rugged coast of Ireland, but never expected to be entrusted with an angel sent to experience a human life. The family is forced from their home, only to find an unwelcoming America on the brink of civil war. Together their journey leads them to a mountainous Virginia mining estate, Catawba Coal & Iron Company. Their youngest son, Peter, holds a wisdom and light beyond his human appearance in a world that threatens to be his undoing. He must learn to navigate a place where his culture is not always welcome, a love foretold by Irish myth, and a country where race dictates who is enslaved and who is free.

Chasing Dreams

release date: Oct 06, 2013
Chasing Dreams
Cooper Krenshaw knows what it''s like to feel the bright lights upon him, the sound of the crowd roaring when he walks on stage; after all, he happens to be one of the biggest names in country music at the moment. And he has knowingly just dropped off the grid. The first decision he has made on his own in quite some time. Cooper only thought he was looking for a challenge when he walked off of that concert stage in Chicago. After finding himself in Devil''s Bend, Texas, he soon realizes that the dream he''s been chasing might not be the one he was after all along. Now he''s found himself not only starting over in a new town, but he''s pretty sure he''s met the toughest challenge of his life... she just happens to be an angel in blue jeans. Tessa Donovan hasn''t been the best of friends with Fate for quite some time now. Owning and operating The Rusty Nail, a small bar in her hometown of Devil''s Bend, has been enough to keep her going through the motions of her life. But it''s the only thing she''s got. After losing her husband fifteen short months after they were married, Tessa has spent the last four years chasing dreams in order to maintain her sanity. Only those dreams are soon ripped right out of her grasp by a sexy country music star who claims he descended on her small town by accident. Coincidence? Or has Fate stepped in once again? Whatever the case, Tessa knows the handsome cowboy might get to overtake her dreams, but she''s bound and determined that he won''t take her heart.

Hot Chocolate Wishes

release date: Nov 30, 2023
Hot Chocolate Wishes
See what''s happening at Christmas in Coyote Ridge with never-before-seen characters who are proving that the holidays are even better when they''re naughty. Reilly Jameson has had a crush on her older brother''s best friend for as long as she can remember. This year, she''s decided to put it all on the line and see if she can give Brady McCord a nudge in the right direction. She intends to show him that despite the age difference, they can be really good together. Donovan Jameson has shrugged off his attraction to his little sister''s best friend for quite some time because Tate Riggs is not his type. But for some reason, he can''t shake it anymore. When Tate calls him sweetie, Donovan wants nothing more than to dominate the younger man and show him just how sweet they can be together. This is a full-length holiday romance with HEAs for two separate couples. Since their lives are so intertwined, it seemed more fun to let it play out side by side.

Naughty Holidays 2016

release date: Dec 01, 2016
Naughty Holidays 2016
holidays, Christmas, New Year''s, Thanksgiving, gay romance, novella, short story

Chase

release date: Jun 28, 2023
Chase
Volume 1: What twenty-four-year-old recent college graduate would sign up to uncover a secret kink club rumored to be hidden within the walls of a multi-billion dollar conglomerate? Me. Why? Well, that''s easy. I''ve spent my entire life with my nose to the grindstone, doing the right thing, focusing on my future, and now I''m bored, which isn''t a good thing when you have an insatiable curiosity. I probably should''ve considered the type of bait needed for a fishing expedition of this magnitude when I applied for the job at Primal Instincts, LLC, looking to uncover the secret world that exists only on the whispers of those who''ve never so much as glimpsed the dark, seductive inner workings because those who had wouldn''t dare reveal anything. Now that I''m through the door, I''ve got my determination and single-minded focus to keep me moving forward. I will figure it out if for no other reason than to say I did. Provided I don''t get sidetracked by not one, not two, but three men who are ridiculously good at keeping secrets. Volume 2: I am only one week into my new job at Primal Instincts, LLC when I realize super sleuth is not something I will be adding to my resume anytime soon. Although I''m still on my mission to uncover the truth about the secret kink club I suspect exists and is somehow tied to my employer, I find myself with more pressing things to deal with than solving that mystery. Between balancing the work assignment I''ve been given and the change in my current dating status, the only time I have left is spent dealing with a dominating, manipulative, not to mention sexy, man who has me in his crosshairs. Unfortunately, the aforementioned dominating man starts the dominoes falling when his manipulation puts me in a vulnerable position. It''s crazy what can happen in a week. Volume 3: To say things have gotten complicated with the men in my life would be an understatement. My mission to discover the secret kink club has been put on hold, but not because I''m distracted by the three men plaguing my thoughts and dreams. Not only because of them, anyway. They are certainly responsible for keeping my attention away from my goals. However, my main concern is the man blackmailing me with a video I''d prefer isn''t seen by the public. That man has given me an ultimatum, and I have a decision to make-an important one. But for the moment, Creed, Hawk, and Garrison are doing their best to keep my mind off my problem, and they have a proposition for me. One that will effectively shift my focus yet again. Note from the author: The Primal Instincts series was originally released in individual volumes. Chase, Capture, and Claim are the combination of these volumes. The stories are the same.

The Optimization of Core Size, Uniformity, and Dye Conjugation of Spherical Substates for Nitric Oxide Sensitive Fluorescent Nanobiosensors

release date: Jan 01, 2012

A Neutral Network Approach to Automated Classification of Cracks in Images of Highway Pavement Using Vectorized Data

release date: Jan 01, 1999

The Modern Jazz Quartet

release date: Jan 01, 2002

The Problem with a One-size-fits-all Approach to Discipline

release date: Jan 01, 2010
The Problem with a One-size-fits-all Approach to Discipline
This thesis serves as a reflective, yet critical review of the author''s use of punitive discipline techniques. The techniques used were coercion, punishments, and rewards which mirrored a zero tolerance, one-size-fits-all approach to disciplining challenging students.

Relationships Between Telomeric Sequences and Structures, DNA Replication, and the Function of the Werner Syndrome Protein

release date: Jan 01, 2012

Rooting for Truth in Humor

release date: Jan 01, 2012

Sydney Hi' De0 -US

release date: Mar 08, 2017
Sydney Hi' De0 -US
"A tale of the art world and all who dwell therein including shapeshifters, ghosts and the occasional demon. A love story where two artists try to win the love of a very beautiful girl indeed, by putting on exhibitions in order to win her attentions, and the outrageous occult repercussions all round. THIS BOOK IS HIGHLY UNLIKELY TO EVER BE OFFICIALLY PUBLISHED BY THE ARTS/LITERARY ESTABLISHMENTS, but either way it is a well written romp as well as being also a tough, hard-bitten, critical/social satire on the world of art culture." - NYT Since most of my books have been downloaded to near death by just about everyone I must make my many avid readers aware that when you download some writers work illegally this means impoverishment unless they have a big, rich publisher who sells lots of their books like jk rowling. I''m not one of these and desperately need the money from them so don''t be evil and steal from the poor artist writer -that''s all folks!

Developing a Human Cell Model for Fragile X Syndrome Using Direct Reprogramming

release date: Jan 01, 2017
Developing a Human Cell Model for Fragile X Syndrome Using Direct Reprogramming
A comprehensive view of the mechanisms underpinning the neurodevelopmental disorder Fragile X Syndrome (FXS) is lacking owing to limited access to relevant human cell models. Therefore, the development of tractable and representative models of human neurodevelopmental disorders is crucial to our ability to draw physiologically relevant conclusions about the mechanisms of FXS pathogenesis. Ideally, experimental models of this disorder would recapitulate the cellular and molecular mechanisms underlying the phenotypic characteristics of FXS as they are manifested in humans. Additionally, both the genetic and phenotypic characteristics of this disorder would be represented within FXS-associated cell types at defined stages of neuronal development that are relevant to FXS progression. The use of cell reprogramming to generate FXS patient-derived human neural stem/precursor cells provides a powerful platform to elucidate the mechanistic underpinnings of aberrant neurodevelopmental phenotypes as they occur in humans. Therefore, the aim of this study was to establish our capability to generate FXS patientderived neural progenitor cells (iNPs) and subsequently neurons by direct reprogramming to a neural progenitor state. Furthermore, direct reprogramming of FXS patient-derived fibroblasts would be achieved using a novel chemically modified mRNA gene delivery system advanced by the Neural Reprogramming and Repair laboratory. This study demonstrated that diverse cell populations positive for both neural progenitor and neuronal markers can be derived directly from somatic cells by SOX2/PAX6-SNIM® RNA mediated reprogramming. Importantly, reprogrammed FXS cell lines exhibited transcriptional and phenotypic differences, which may be suggestive of early FXS-associated mechanisms occurring during progenitor and neuronal development in vitro. This is significant as it provides proof-of-concept data that this model may be useful in future efforts to characterise the effects of the loss of FMRP on the development and function of neurons and uncover possible therapeutic targets for early intervention strategies in the future. To the best of our knowledge, the present study is the first to derive live human neural progenitors and differentiated neuron-like cells by direct-to-iNP reprogramming of FXS-patient derived somatic cells.
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