New Releases by Brendan James

Information divulged during a seemingly innocent conversation on board an overnight ferry to Spain results in a situation which snowballs out of control, especially when greed rears its ugly head. But who polices crime at sea? And why does a policeman from the Metropolitan Police become involved?

Any task requires a strategy and learning a foreign language is no exception. By following the advice in The Second Language Learner''s Handbook, you should be able to achieve your objectives faster.

Tom Gibbs is about to take a two week annual holiday from the mansion house where he is employed. As usual, he needs to arrange a replacement security guard to cover his duties while he is away. The employment agency sends Martin Phillips. Tom escorts Martin on a tour of the house to show him the ropes. All is well until they reach the cellar, where events take a dramatic turn.

After yet another failed relationship, and heading towards middle age, John is left wondering why he has so much bad luck with women. He begins to look back over his life and realizes that it could be him that is the problem, and not his girlfriends. After much deliberation, he reaches a conclusion and decides to make a drastic change to his personal life. He goes to visit his gay friend Bob to make an announcement and to ask if he can help in some way.

Stephen and Claire have decided to live in a caravan while their new house is being built by Stickings, a local builder. Everything appears normal until Stickings makes a gruesome discovery during the excavation works

Mitochondria are essential for nearly all eukaryotic cells and play a key role in more than 300 human diseases. Over the past several decades, in part due to advancements in proteomics and the integration of orthogonal approaches, the components of the mitochondrial proteome have been revealed to near completion. In contrast to this depth of knowledge, we find that 20% of the mitochondrial proteome is comprised of uncharacterized, orphan mitochondrial proteins (OMPs). Similarly, many patients with overt mitochondrial disease lack molecular diagnoses. To illuminate key areas of mitochondrial biology, we apply mass spectrometry-based proteomics to quantify the dynamic nature of mitochondrial post-translational modifications (PTMs) and protein-protein interactions (PPIs). While the known diversity and prevalence of mitochondrial protein PTMs have expanded rapidly of late, the regulatory role of these modifications, if any, is still poorly understood. To evaluate the extent and importance of one such PTM, lysine acetylation, we quantified the mouse liver mitochondrial acetylproteome under two pairs of contrasting states - obese vs. lean and fasted vs. refed. Our proteomic analyses support the identification of thousands of acetyl sites on hundreds of proteins. Acetyl abundance at more than 500 sites is dynamically regulated across the aforementioned conditions. Furthermore, we apply state-of-the-art protein purification techniques to establish the inhibitory capacity of acetylation of the key metabolic enzyme Acat1 at each of two dynamically-acetylated active site lysine residues. To accelerate the functional characterization of the more than 200 orphan mitochondrial proteins, we applied affinity enrichment coupled to mass spectrometry (AE-MS) to map OMP-related PPIs across two cell types and nutrient states. We establish the first unbiased evidence of a mammalian lipid biosynthetic complex involving several OMPs. Furthermore, we associate many OMPs with known pathways and processes for the first time. In this work, we evaluate the current state of mitochondrial protein and disease annotation, we expand the understanding of the regulatory nature of mitochondrial protein acetylation, we find evidence for and map the architecture of a mammalian lipid biosynthetic complex, and we accelerate the functional annotation of more than a dozen OMPs.

National trade preference schemes are an integral component of North-South trade relations. Yet the varying degree of trade liberalization across different preference schemes is considerable, especially in the case of the EU. This thesis attempts to find what factors can account for the differing amount of trade liberalization between the EU''s trade preference schemes. To do this, both process tracing and the congruence method are used to analyze two different instances of European trade and development policy--the EU''s 2012 revision of GSP and the EU''s Economic Partnership Agreement with the Caribbean. I find the liberalization of EU trade preferences is driven primarily by three factors: (1) Domestic commercial interests; (2) EU concerns about global economic competitiveness; and (3) Desires for WTO compatibility. I also find NGOs to represent the main opposition for the EU''s current trade and development agenda rather than import-competing sectors as "conventional" trade theory would predict.

Hearing impairment affects as many as 15% of the U.S. population. Effective audiological rehabilitation for individuals with hearing loss requires amplification of auditory stimuli via the use of hearing aids that have been properly fitted by a hearing health professional; however many people who are fitted for hearing aids do not use them regularly. Investigators evaluate the effect of a reinforcer sampling procedure in two concurrent multiple baseline experiments with individuals on two dependent variables related to hearing aid usage: (a) daily hearing aid usage by participants in hours per day (Experiments 1 and 2) and (b) the proportion of time that hearing aids were used by participants in acoustical environments that were similar to the acoustical environment present during the reinforcer sampling procedure (Experiment 1). Hearing aid usage, automatically recorded via datalogging features, is introduced as a behavioral cusp skill for individuals whose normal communication abilities are impaired by hearing loss. The use of hearing aids is identified as a socially significant behavior affecting a large and growing population of hearing impaired individuals currently underserved by applied behavior analysis researchers and practitioners.

Intellectual property holders have invested significant resources into Second Life in pursuit of customers and virtual market share. 15.8 million residents are available to hear your message. Within this virtual world, however, they have found age old intellectual property problems: counterfeiters with virtual Herman Miller furniture and Rolex watches.Similarly, participants have become residents of Second Life for the opportunity to develop virtual intellectual property.Virtual art galleries, magazines, and bar associations have sprung up. The United States Patent and Trademark Office has grantedregistration to virtual trademarks created within Second Life.Both real world and virtual world intellectual property holders have come to Linden Research''s Second Life on the premise that their intellectual property would be protected. Is it? How should the added exposure of a virtual intellectual property portfolio be managed by an intellectual property right holder?Recently filed complaints indicate that Linden Lab''s “commercially reasonable efforts” may not be sufficient. In fact, they may be detrimental to the intellectual property owner. Here, I will discuss suits filed against Linden Lab regarding Second Life''s intellectual property protection. The complaints are illustrative of the peril in having intellectual property rights be privately adjudicated and enforced.

This is the extension of project involving educating a local corporation on Payment Card Industry Data Security Standard compliance, widely known as "PCI compliance." PCI compliance is a matter of adhering to the pseudo-regulatory standard of a consortium of payment card companies. Any company which accepts payment cards - including the ubiquitous credit card - must be compliant or face fines from the payment card companies and liability in the event of a data breach. This is the written result of this project, made anonymous. It makes the business case for PCI compliance, and then explains the mechanics of attaining compliance. This article is suitable for any organization that has an eCommerce front that they outsource. Doing so significantly reduces their exposure, as is explained.

Glycosaminoglycans are linear polysaccharides that decorate the surfaces of all animal cells. The heterogeneity of the polysaccharide, especially the pattern of sulfation on the distal end of the chain, endows these glycans with diverse biological functions including essential roles in signaling during animal development. Thus, glycosaminoglycans represent an extremely important and interesting class of glycans to probe using metabolic labeling in concert with bioorthogonal chemistry. This thesis describes the extension of the metabolic labeling strategy to glycosaminoglycans (GAGs). Prior to the work described herein, GAGs could not be metabolically labeled because xylose, the monosaccharide specific to these glycans, lacks a salvage or biosynthetic pathway capable of converting xylose derivatives, or other simple sugars, to the ultimate sugar donor UDP-xylose. In order to label GAGs, the UDP-xylose derivative itself must be delivered to cells. However, the charged nature of nucleotide sugars prevents passive diffusion through hydrophobic membranes, and eukaryotic cells lack a plasma membrane nucleotide sugar transporter to provide facilitated or active transport. Prior research in our lab, however, had demonstrated that unnatural nucleotide sugars could be delivered to cells by microinjection into the yolk sacs of developing zebrafish embryos. In addition to providing a tractable solution to deliver nucleotide sugars, zebrafish are a well-established vertebrate development model organism with excellent properties for molecular imaging. It was hypothesized that GAGs could be metabolically labeled by microinjecting unnatural variants of UDP-xylose into zebrafish embryos. To this end, three analogs of UDP-xylose were synthesized, microinjected into zebrafish embryos, and analyzed for incorporation onto zebrafish embryo cell surfaces by reaction with fluorescent cyclooctyne probes. Two of the analogs, UDP-2-XylAz and UDP-3-XylAz, did not provide azide-dependent labeling. UDP-4-XylAz did provide azide-dependent labeling, and the azide replacing the C-4 hydroxyl group inhibits extension of the GAG polysaccharide beyond the initial xylose unit. Therefore, UDP-4-XylAz functions as a selective metabolic inhibitor of GAG biosynthesis. The phenotypic consequences of aberrant GAG production were ascertained and these results add to our understanding of the importance of GAGs during normal vertebrate development.

Currently, virtual world governance is the product of end user license agreements, contracts which attempt to provide a complete legal and enforcement system to the virtual world. This method of governance is flawed, however, and results in participant frustration. Alternative approaches that have been advanced so far include governmental regulation, which has begun in some countries. However, numerous pressures and precedent resist such an application in the United States. The following argues against keeping just the license agreements as the body of law, and also against a wholesale shift toward governmental regulation.Instead, a compromise -- establishing a standards-setting body of developers, referred to here as a virtual world council -- is the most efficient solution. This is a daunting task; however, numerous examples exist for the developers of virtual worlds to follow and they are explained below. The final proposed result is a two-tier system that retains EULAs with modifications as appropriate, and supports a common law approach rooted in standardization for resolving what EULAs cannot.

Globally it is estimated that 35 million people were living with HIV at the end of 2012. Unprotected sexual transmission of HIV accounts for the majority of all new infections, with semen acting as a vector. HIV levels in blood of an HIV-infected individual is the best-defined predictor of transmission risk, however, as blood is not often a factor during sex, this association likely reflects the level of HIV RNA in semen. It has been shown that while blood viral load reaches a set point during chronic untreated infection, semen can remain highly variable. Factors responsible for this heterogeneity have not fully well characterized. Furthermore, while highly active antiretroviral therapy has been associated with suppression of blood and genital viral loads, some men continue to shed HIV in their semen, despite undetectable viremia, a phenomenon called isolated semen HIV RNA shedding (IHS). In my doctoral thesis I evaluated the impact of semen collection method on viral load measurements, correlations between antiretroviral therapy intensification and its effect on immune activation, viral load and IHS as well as factors contributing to semen viral load variability in drug naive men. Overall, I found that semen collected directly into transport medium had a large impact on the measurement of viral load, evidencing a 0.42log10 increase in viral load over neat collection. Initiation of intensified antiretroviral therapy was shown to decrease viral load in blood and semen faster than a standard regimen. Intensification, however, did not resolve the occurrence of ISH, nor did it reduce systemic immune activation faster then a conventional therapy. It was observed that men on longer durations of ART had no evidence of IHS, suggesting the phenomenon is transient and may resolve with longer duration of therapy. Finally I explored factors that may be linked to the variability seen in semen viral load in drug naive men. Of all parameters examined, the macrophage-associated cytokine IL-8 was the only factor independently predictive of semen viral load (aside from blood viral load) in longitudinal studies. This suggests IL-8 as a common pathway linking clinical causes of high semen HIV levels in therapy-naive men.

This article details strategies to build a nonprofit corporation dedicated to affordable housing development. The intended audiences are prospective nonprofit corporation organizers, nonprofit housing developers, and those interested in learning more about nonprofit affordable housing development regardless of their background.

Inspired by the recent interest in using geothermal energy from the Perth Basin, models of the underground water ow in the deep sedimentary basin are being developed. If convection were to occur, then a circulation of uid assists in bringing hot water closer to the surface. If such hot upwellings can be located, the cost of extracting water at an operational temperature can be reduced. This thesis explores issues of modelling convection in porous media, with particular focus on the e ect of the domain size on the observed convection patterns. Convection in an in nite layer of a porous medium occurs if the dimensionless Rayleigh number exceeds a critical value. This is also true for a box of a porous medium, however, each discrete modal solution has its own associated critical Rayleigh number. Usually just one mode will be generated at the onset of convection, however, there are many critical box dimensions for which up to four modes share the same critical Rayleigh number and all may be generated at the onset of convection. In such circumstances there will be a slow interchange of energy between the preferred modes. A perturbation method is applied to a system where multiple modes are generated at onset to yield a system of ordinary di erential equations which govern the evolution of the amplitudes of the viable modes. For three interacting modes, three unique cases arise, each with a di erent phase-space structure. Critical boxes with "moderate" aspect ratios are systematically categorised into these cases. While two of the examples represent the usual case where just one mode survives in the nal state, the third example is a special case where it is possible for the three modes to coexist. This procedure is extended to a case where four modes are generated at the onset of convection, completing all possible scenarios in a moderate box domain. The initial conditions determine which mode(s) will survive. For non-critical boxes, the bifurcations that occur as the Rayleigh number increases are analysed and pro led in the weakly-nonlinear regime.

"... reviews current knowledge on the impacts on yield of widening row spacing for crops including wheat, barley, canola and lupins in southern Australia. Widening of row spacing has accompanied the adoption of conservation cropping systems with no-till and stubble retention. Row space widening has allowed sowing machinery to operate in stubble with minimal blockages caused by the retained stubble."--Foreword.

This book is a collector''s guide for all Pink Floyd fans around the world. Included you will find track listings on more than 800 vinyl albums, vinyl singles, CD albums, CD singles, some promos, 8-Track tapes, Cassette tapes. Most release dates and catalog numbers are listed, with recordings released from more than 25 countries included. B & W cover art illustrations throughout the book. is a must have guide for any Pink Floyd Fan!

Through a study of its natural environment and irrigation system, this dissertation investigates the evolution of the landscape of Egypt''s Fayyum depression across sixteen centuries, from the third century BCE to the thirteenth century CE. From the evidence of Greek papyri, Arabic fiscal documentation, early modern travel literature, archaeology, and contemporary scientific work, I chart the changes in human relationships with earth and water over time, changes which constantly altered the inhabited and cultivated regions of the Fayyum. My main argument throughout is that it was local agency and not state governments that continuously remade the landscape. The history of the Fayyum after the fourth century CE has long been viewed by ancient historians as one of decline from its ancient heights due to the failure of the late Roman and Muslim successor states to properly manage its irrigation system. I locate the genesis of this narrative within nineteenth century perceptions of the docility of nature and the belief that ancient governments had achieved centralized control over the Nile and the Egyptian environment. This anachronistic retrojection of the characteristics of the modern irrigation system has had a considerable afterlife in historical scholarship on Egyptian irrigation. Eschewing a narrow focus on the state, this dissertation argues that that nature is a potent agent in its own right. Ancient farmers could not control nature so they adapted to it, creating four distinct irrigated sub-regions in the Graeco-Roman Fayyum, each tailored to the particulars of the local environment. Our papyri stem from only one of these sub-regions, the water-scarce margins, which lay at the tail end of the irrigation system. Here, inadequate irrigation and fertilization progressively led to soil salinization and degradation, which helped to spur the eventual abandonment of these areas. By the medieval period, only the central floodplain remained inhabited. Only here was sustainable agriculture under the regime of premodern technology possible. Although the Roman state coordinated local labor on the canals, nothing could bind Fayyum villagers to the degrading margins in perpetuity. Fourth century papyri hint that some cultivators had moved to other nomes and were prospering. Still later documents of the sixth to eighth centuries CE reveal greatly increased settlement density in the central Fayyum. Thus, it was local cultivators who made and remade the landscape of the Fayyum over the centuries according to their own needs. Government could both guide and benefit from this local labor but it could never fully control it.

This book is a collector''s guide for all King Crimson fans around the world. Included you will find track listings on more than 800 vinyl albums, vinyl 7" and 12" singles, CD albums, CD singles, some promos, 8-Track tapes, Cassette Tapes and even a Reel to Reel tape. Most release dates and catalog numbers are listed, with recordings released from more than 25 countries included. There are more than 110 b & w cover art illustrations throughout the book. An unauthorized releases section is included which list many of the bootleg, counterfeits and pirated records from around the world. We have also included a section that lists album credits / details for 90 King Crimson releases. This is a must have guide for any King Crimson Fan!

A list of ''must-play'' golf courses in Australia and New Zealand.